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This research was supported by the Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria (INIA) (grants RTA2012-00097, RTA2015-00046, and RFP2015-00015) and the Gobierno de Aragon- European Social Fund, European Union (Grupo Consolidado A12).

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Mapping Quantitative Trait Loci Associated With Graft (In)Compatibility in Apricot (Prunus armeniaca L.)

Publicado en:Frontiers In Plant Science. 12 (622906): 622906- - 2021-02-19 12(622906), DOI: 10.3389/fpls.2021.622906

Autores: Pina, Ana; Irisarri, Patricia; Errea, Pilar; Zhebentyayeva, Tetyana

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Graft incompatibility (GI) between the most popular Prunus rootstocks and apricot cultivars is one of the major problems for rootstock usage and improvement. Failure in producing long-leaving healthy grafts greatly affects the range of available Prunus rootstocks for apricot cultivation. Despite recent advances related to the molecular mechanisms of a graft-union formation between rootstock and scion, information on genetic control of this trait in woody plants is essentially missing because of a lack of hybrid crosses, segregating for the trait. In this study, we have employed the next-generation sequencing technology to generate the single-nucleotide polymorphism (SNP) markers and construct parental linkage maps for an apricot F-1 population Moniqui (Mo) x Paviot (Pa) segregating for ability to form successful grafts with universal Prunus rootstock Marianna 2624. To localize genomic regions associated with this trait, we genotyped 138 individuals from the Mo x Pa cross and constructed medium-saturated genetic maps. The female Mo and male Pa maps were composed of 557 and 501 SNPs and organized in eight linkage groups that covered 780.2 and 690.4 cM of genetic distance, respectively. Parental maps were aligned to the Prunus persica v2.0 genome and revealed a high colinearity with the Prunus reference map. Two-year phenotypic data for characters associated with unsuccessful grafting such as necrotic line (NL), bark and wood discontinuities (BD and WD), and an overall estimate of graft (in)compatibility (GI) were collected for mapping quantitative trait loci (QTLs) on both parental maps. On the map of the graft-compatible parent Pa, two genomic regions on LG5 (44.9-60.8 cM) and LG8 (33.2-39.2 cM) were associated with graft (in)compatibility characters at different significance level, depending on phenotypic dataset. Of these, the LG8 QTL interval was most consistent between the years and supported by two significant and two putative QTLs. To our best knowledge, this is the first report on QTLs for graft (in)compatibility in woody plants. Results of this work will provide a valuable genomic resource for apricot breeding programs and facilitate future efforts focused on candidate genes discovery for graft (in)compatibility in apricot and other Prunus species.

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