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Publicaciones > Artículo

Vaginal Microbiota Is Stable throughout the Estrous Cycle in Arabian Maress

Publicado en:Animals. 10 (11): 1-14 - 2020-11-01 10(11), doi: 10.3390/ani10112020

Barba M.;

Afiliaciones

Cardenal Herrera CEU Univ, Res Grp Microbiol Agents Associated Anim Reprod P, Dept Anim Prod & Hlth Vet Publ Hlth & Food Sci &, Fac Vet Med,CEU Univ, Alfara Del Patriarca 46115, Spain - Autor o Coautor
Centro de Investigación y Tecnología Agroalimentaria de Aragón - Entidad de origen
Micro Syst SL, Barcelona 08003, Spain - Autor o Coautor
Microomics Systems S.L - Autor o Coautor
Ministerio de Ciencia, Innovación y Universidades - Financiador
Universidad Cardenal Herrera-CEU - Autor o Coautor
Universidad CEU Cardenal Herrera - Entidad de origen
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Resúmen

Resúmen: © 2020 by the authors. Licensee MDPI, Basel, Switzerland. Lactic acid bacteria (LAB) dominate human vaginal microbiota and inhibit pathogen proliferation. In other mammals, LAB do not dominate vaginal microbiota, however shifts of dominant microorganisms occur during ovarian cycle. The study objectives were to characterize equine vaginal microbiota in mares by culture-dependent and independent methods and to describe its variation in estrus and diestrus. Vaginal swabs from 8 healthy adult Arabian mares were obtained in estrus and diestrus. For culture-dependent processing, bacteria were isolated on Columbia blood agar (BA) and Man Rogosa Sharpe (MRS) agar. LAB comprised only 2% of total bacterial isolates and were not related to ovarian phases. For culture-independent processing, V3/V4 variable regions of the 16S ribosomal RNA gene were amplified and sequenced using Illumina Miseq. The diversity and composition of the vaginal microbiota did not change during the estrous cycle. Core equine vaginal microbiome consisted of Firmicutes, Bacteroidetes, Proteobacteria and Actinobacteria at the phylum level. At the genus level it was defined by Porphyromonas, Campylobacter, Arcanobacterium, Corynebacterium, Streptococcus, Fusobacterium, uncultured Kiritimatiaellae and Akkermansia. Lactobacillus comprised only 0.18% of the taxonomic composition in estrus and 0.37% in diestrus. No differences in the relative abundance of the most abundant phylum or genera were observed between estrus and diestrus samples.

Palabras clave: Diestrus; Equine; Estrus; Horses; Metagenomic; Microbiome; Reproductive; Vagina

Indicios de calidad

WoS Scopus by Scimago
Best Categ.Agriculture, Dairy & Animal ScienceVeterinary (Miscellaneous)
WoSScopus by ScimagoSPIFecytAgaurDialnetCircCapesMiar
IF2.7520.584--10.500
Rank13/6148/207
QQ1Q1-----A2
DD2D3-
TT1T1
PP80P24
Index ERIC-
Index Emergin-
Index AHCI-