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Publicaciones > Article

Vaginal Microbiota Is Stable throughout the Estrous Cycle in Arabian Maress

Publicated to:Animals. 10 (11): 1-14 - 2020-11-01 10(11), doi: 10.3390/ani10112020

Barba M.;

Affiliations

Cardenal Herrera CEU Univ, Res Grp Microbiol Agents Associated Anim Reprod P, Dept Anim Prod & Hlth Vet Publ Hlth & Food Sci &, Fac Vet Med,CEU Univ, Alfara Del Patriarca 46115, Spain - Author
Centro de Investigación y Tecnología Agroalimentaria de Aragón - Source entity
Micro Syst SL, Barcelona 08003, Spain - Author
Microomics Systems S.L - Author
Ministerio de Ciencia, Innovación y Universidades - Funding
Universidad Cardenal Herrera-CEU - Author
Universidad CEU Cardenal Herrera - Source entity
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Abstract

Summary: © 2020 by the authors. Licensee MDPI, Basel, Switzerland. Lactic acid bacteria (LAB) dominate human vaginal microbiota and inhibit pathogen proliferation. In other mammals, LAB do not dominate vaginal microbiota, however shifts of dominant microorganisms occur during ovarian cycle. The study objectives were to characterize equine vaginal microbiota in mares by culture-dependent and independent methods and to describe its variation in estrus and diestrus. Vaginal swabs from 8 healthy adult Arabian mares were obtained in estrus and diestrus. For culture-dependent processing, bacteria were isolated on Columbia blood agar (BA) and Man Rogosa Sharpe (MRS) agar. LAB comprised only 2% of total bacterial isolates and were not related to ovarian phases. For culture-independent processing, V3/V4 variable regions of the 16S ribosomal RNA gene were amplified and sequenced using Illumina Miseq. The diversity and composition of the vaginal microbiota did not change during the estrous cycle. Core equine vaginal microbiome consisted of Firmicutes, Bacteroidetes, Proteobacteria and Actinobacteria at the phylum level. At the genus level it was defined by Porphyromonas, Campylobacter, Arcanobacterium, Corynebacterium, Streptococcus, Fusobacterium, uncultured Kiritimatiaellae and Akkermansia. Lactobacillus comprised only 0.18% of the taxonomic composition in estrus and 0.37% in diestrus. No differences in the relative abundance of the most abundant phylum or genera were observed between estrus and diestrus samples.

Keywords: Diestrus; Equine; Estrus; Horses; Metagenomic; Microbiome; Reproductive; Vagina

Quality index

WoS Scopus by Scimago
Best Categ.Agriculture, Dairy & Animal ScienceVeterinary (Miscellaneous)
WoSScopus by ScimagoSPIFecytAgaurDialnetCircCapesMiar
IF2.7520.584--10.500
Rank13/6148/207
QQ1Q1-----A2
DD2D3-
TT1T1
PP80P24
Index ERIC-
Index Emergin-
Index AHCI-