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This research was supported in part by the Spanish Ministerio de Ciencia, Innovacion y Universidades, co-funded with FEDER (AGL2017-85563-C2-2-R) and partially financed by Aragon Government grant for Research group, A11-20R-PROVESOS.

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Garces-Claver, AnaAuthor
Publicaciones > Article

Assessing the Genetic Diversity and Population Structure of a Tunisian Melon (Cucumis melo L.) Collection Using Phenotypic Traits and SSR Molecular Markers

Publicated to:Agronomy-Basel. 11 (6): - 2021-06-01 11(6), doi: 10.3390/agronomy11061121

Garces-Claver, Ana;


A11-20R-PROVESOS FEDER - Funding
AGL2017-85563-C2-2-R Aragon Government - Funding
AGL2017-85563-C2-2-R Spanish Ministerio de Ciencia, Innovacion y Universidades - Funding
Centro de Investigación y Tecnología Agroalimentaria de Aragón - Source entity
Natl Gene Bank Tunisia, Blvd Leader Yasser Arafat, Tunis 1080, Tunisia - Author
TELUQ Univ, LICEF Res Ctr, 5800 Rue St Denis, Quebec City, PQ H2S 3L5, Canada - Author
Univ Sousse, High Inst Agron Chott Mariem, Res Unit Organ & Convent Vegetable Crops UR13AGR0, Sousse 4042, Tunisia - Author
Univ Sousse, Reg Res Ctr Hort & Organ Agr CRRHAB IRESA, LR21AGR03, Sousse 4042, Tunisia - Author
Univ Zaragoza, CITA, Inst Agroalimentario Aragon IA2, Ctr Invest & Tecnol Agroalimentaria Aragon, Zaragoza 50059, Spain - Author
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Summary: The assessment of genetic diversity and structure of a gene pool is a prerequisite for efficient organization, conservation, and utilization for crop improvement. This study evaluated the genetic diversity and population structure of 24 Tunisian melon accessions, by using 24 phenotypic traits and eight microsatellite (SSR) markers. A considerable phenotypic diversity among accessions was observed for many characters including those related to agronomical performance. All the microsatellites were polymorphic and detected 30 distinct alleles with a moderate (0.43) polymorphic information content. Shannon's diversity index (0.82) showed a high degree of polymorphism between melon genotypes. The observed heterozygosity (0.10) was less than the expected heterozygosity (0.12), displaying a deficit in heterozygosity because of selection pressure. Molecular clustering and structure analyses based on SSRs separated melon accessions into five groups and showed an intermixed genetic structure between landraces and breeding lines belonging to the different botanical groups. Phenotypic clustering separated the accessions into two main clusters belonging to sweet and non-sweet melon; however, a more precise clustering among inodorus, cantalupensis, and reticulatus subgroups was obtained using combined phenotypic-molecular data. The discordance between phenotypic and molecular data was confirmed by a negative correlation (r = -0.16, p = 0.06) as revealed by the Mantel test. Despite these differences, both markers provided important information about the diversity of the melon germplasm, allowing the correct use of these accessions in future breeding programs. Together they provide a powerful tool for future agricultural and conservation tasks.

Keywords: Accessions; Breeding lines; Characters; Flexuosus; Genetic diversity; Germplasm; Landraces; Linkage map; Molecular markers; Phenotypic traits; Rapd; Sequence; Snake melon

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