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This study was supported by the Ministry of Economy and Competitiveness of Spain, the European Union Regional Development Funds (INIA RTA2012-080-00, INIA RZP2017-00001-00) and the Research Group Funds of Aragon Government (A14_17R).

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Blanco MAuthorJoy MAuthorCalvo JhCorresponding Author
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Publicaciones > Article

Gene Expression and Fatty Acid Profiling in Longissimus thoracis Muscle, Subcutaneous Fat, and Liver of Light Lambs in Response to Concentrate or Alfalfa Grazing

Publicated to:Frontiers In Genetics. 10 (1070): 1070-1070 - 2019-01-01 10(1070), doi: 10.3389/fgene.2019.01070

Blanco M; Joy M; Calvo Jh;

Affiliations

ARAID, Zaragoza, Spain - Author
Centro de Investigación y Tecnología Agroalimentaria de Aragón - Source entity
IMDEA Alimentac, Precis Nutr & Obes, Madrid, Spain - Author
INIA, Dept Mejora Genet Anim, Madrid, Spain - Author
Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA - Author
Univ Alberta, Livestock Gentec, Edmonton, AB, Canada - Author
Univ Zaragoza, Unidad Prod & Sanidad Anim, Ctr Invest & Tecnol Agroalimentaria Aragon, IA2,CITA, Zaragoza, Spain - Author
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Abstract

Summary: A better understanding of gene expression and metabolic pathways in response to a feeding system is critical for identifying key physiological processes and genes associated with polyunsaturated fatty acid (PUFA) content in lamb meat. The main objective of this study was to investigate transcriptional changes in L. thoracis (LT) muscle, liver, and subcutaneous fat (SF) of lambs that grazed alfalfa (ALF) and concentrate-fed (CON) slaughtered at 23 kg and using the Affymetrix Ovine Gene 1.1 ST whole-genome array. The study also evaluated the relationship between meat traits in LT muscle, including color, pigments and lipid oxidation during 7 days of display, alpha-tocopherol content, intramuscular fat (IMF) content and the fatty acid (FA) profile. Lambs that grazed on alfalfa had a greater alpha-tocopherol concentration in plasma than CON lambs (P < 0.05). The treatment did not affect the IMF content, meat color or pigments (P > 0.05). Grazing increased the alpha-tocopherol content (P < 0.001) and decreased lipid oxidation on day 7 of display (P < 0.05) in LT muscle. The ALF group contained a greater amount of conjugated linoleic acid (CLA), C18:3 n-3, C20:5 n-3, C22:5 n-3, and C22:6 n-3 than did the CON group (P < 0.05). We identified 41, 96 and four genes differentially expressed in LT muscle, liver, and subcutaneous fat, respectively. The most enriched biological processes in LT muscle were skeletal muscle tissue development, being the genes related to catabolic and lipid processes downregulated, except for CPT1B, which was upregulated in the ALF lambs. Animals grazing alfalfa had lower expression of desaturase enzymes in the liver (FADS1 and FADS2), which regulate unsaturation of fatty acids and are directly involved in the metabolism of n-3 PUFA series. The results found in the current study showed that ingesting diets richer in n-3 PUFA might have negative effects on the de novo synthesis of n-3 PUFA by downregulating the FADS1 and FADS2 expression. However, feeding diets poorer in n-3 PUFA can promote fatty acid desaturation, which makes these two genes attractive candidates for altering the content of PUFAs in meat.

Keywords: Alfalfa; Carcass; Color; Concentrate; Feeding system; Finishing period length; Lipid oxidation; Liver; Meat quality; Meat tenderness; Microarray; Muscle; Myostatin; Ovine; Rapid method; Sheep; Subcutaneous fat; Vitamin-e supplementation

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WoS Scopus by Scimago
Best Categ.Genetics & HeredityMolecular Medicine
WoSScopus by ScimagoSPIFecytAgaurDialnetCircCapesMiar
IF3.2601.469--10.500
Rank73/17537/163
QQ2Q1-----A1
DD5D3-
TT2T1
PP58P23
Index ERIC-
Index Emergin-
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