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Ripoll, GuillermoAuthor

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March 16, 2026
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Reduced Nitrates/Nitrites and Natural Additives in Organic Fermented Dry‐Cured Sausages: Effects on Microbiological Safety, Quality, and Consumer Acceptance

Publicated to: JOURNAL OF FOOD SCIENCE. 91 (3): - 2026-03-14 91(3), DOI: 10.1111/1750-3841.70985

Authors:

Argemí‐Armengol, Immaculada; Ripoll, Guillermo; Villalba, Daniel; Álvarez‐Rodriguez, Javier
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Affiliations

Animal Science Department Centro de Investigación y Tecnología Agroalimentaria Aragón (CITA) Zaragoza Spain, Instituto Agroalimentario de Aragón – IA2 (CITA‐Universidad de Zaragoza) Zaragoza Spain - Author
Departament of Animal Science Universitat de Lleida Lleida Spain - Author
Instituto Agroalimentario de Aragón – IA2 (CITA‐Universidad de Zaragoza) Zaragoza Spain, Departamento de Producción Animal y Ciencia de los Alimentos, Escuela Politécnica Superior de Huesca, Carretera de Cuarte s/n University of Zaragoza Huesca Spain - Author
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Abstract

This study examines hydroxytyrosol (HXT), a natural antioxidant, as a partial substitute for curing agents in fermented dry-cured organic sausages. Increasing health concerns regarding nitrates/nitrites, along with EU efforts to reduce their use, highlight the need for alternatives. The effects of partially replacing potassium nitrate or sodium nitrite with HXT were evaluated in terms of physicochemical, microbiological, and sensory properties. Five treatments were produced: C1 (80 mg/kg KNO3), HXT100 (40 mg/kg KNO3 + 100 mg/kg HXT), HXT300 (40 mg/kg KNO3 + 300 mg/kg HXT), C2 (80 mg/kg NaNO2), and HXT200 (40 mg/kg KNO2 + 200 mg/kg HXT). HXT significantly reduced weight loss and lipid oxidation and accelerated the pH drop during early fermentation, potentially enhancing beneficial microbial activity. Proximate composition (moisture, fat, protein, ash) and water activity were unaffected. Sausages containing HXT appeared whiter and brighter (higher L* values). Residual nitrite declined markedly during fermentation across all treatments. HXT100 and HXT300 showed higher Listeria monocytogenes counts than the full-dose control, raising microbial safety concerns. Consumer sensory evaluations (n = 204) favored traditional formulations (C1 and C2) for taste, texture, and overall quality. Interestingly, when informed about the use of natural ingredients, younger and health-conscious consumers rated all samples more positively. These findings suggest that HXT can serve as a clean-label antioxidant in cured meats; however, using it as a complete replacement for nitrates/nitrites may compromise safety. A balanced approach is recommended for developing future organic meat products.
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Keywords

Ácidos grasosAlimentos biológicosCarne de cerdoNitritosPolifenolesProductos de la carne

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal JOURNAL OF FOOD SCIENCE due to its progression and the good impact it has achieved in recent years, according to the agency Scopus (SJR), it has become a reference in its field. In the year of publication of the work, 2026, it was in position , thus managing to position itself as a Q1 (Primer Cuartil), in the category Food Science.

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Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2026-04-05:

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 2.
  • The number of mentions on the social network X (formerly Twitter): 3 (Altmetric).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

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Awards linked to the item

The authors are grateful for the financial support received from the Government of Catalonia and the European Regional Development Funds (BDNS 657748).
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