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Analysis of institutional authors

Fadon, EAuthorRodrigo, JAuthor

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June 13, 2025
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Enzymatic Starch Quantification in Developing Flower Primordia of Sweet Cherry

Publicated to:Bio Protoc. 15 (7): e5256- - 2025-04-05 15(7), DOI: 10.21769/bioprotoc.5256

Authors: Santolaria, N; Fadón, E; Rodrigo, J; Hedhly, A

Affiliations

Fdn Agencia Aragonesa Invest & Desarrollo ARAID - Author
Univ Zaragoza, CITA, Inst Agroalimentario Aragon IA2 - Author

Abstract

Starch is a carbohydrate widely used in the plant kingdom as a fuel for different physiological processes. While different techniques are available for the quantification of starch stored in seeds and bark tissues, they have hardly been used to quantify starch content in developing flower buds, where starch has been reported to accumulate in different reproductive organs. Here, we detail a quantitative enzymatic method to measure starch concentration in developing flower primordia in sweet cherry (Prunus avium L.). First, starch is enzymatically hydrolyzed to D-glucose, which was then quantified by an enzyme-coupled assay involving hexokinase (HK) and glucose-6-phosphate dehydrogenase (G6PD) and spectrophotometric quantification of NADH absorbance at 340 nm. This method is a sensitive, rapid, and affordable protocol specifically optimized for tiny flower buds with low starch content. The technique is revealed to successfully determine starch content in non-freshly harvested samples-frozen and stored at-20 degrees C or stored in fixatives-allowing a temporal separation of sampling and quantification and making the protocol suitable for high-throughput experimental designs in different fields of plant research. Key features center dot Sensitive protocol to quantify starch content in developing flower primordia of sweet cherry. center dot Starch quantification based on enzymatic hydrolysis of starch and spectrophotometric quantification of NADH absorbance at 340 nm using the hexokinase and glucose-6-phosphate dehydrogenase coupled assay. center dot Accurately detects starch in very small samples with low starch concentrations in both fixed and frozen plant material.

Keywords

Análisis enzimáticoApricotÁrboles frutalesEspectrofotometríaFateFlower primordiaHk/g6pd assayMetabolismNadh spectrophotometric assayPrimordios foliaresPrunus aviumReservesStarch enzymatic assaySweet cherryTemperate fruit trees

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Bio Protoc due to its progression and the good impact it has achieved in recent years, according to the agency Scopus (SJR), it has become a reference in its field. In the year of publication of the work, 2025, it was in position , thus managing to position itself as a Q2 (Segundo Cuartil), in the category . Notably, the journal is positioned en el Cuartil Q3 for the agency WoS (JCR) in the category Biology.

Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-07-20:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 2.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 1 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 2.
  • The number of mentions on the social network X (formerly Twitter): 1 (Altmetric).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.
  • Assignment of a Handle/URN as an identifier within the deposit in the Institutional Repository: http://hdl.handle.net/10532/7583

Leadership analysis of institutional authors

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (Santolaria, N) and Last Author (Hedhly, A).

the author responsible for correspondence tasks has been Santolaria, N.