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Grant support

Ministerio de Ciencia, Innovacion y Universidades, Grant/Award Number: PID2022-136478OB-C32; Ministerio de Ciencia e Innovacion, Grant/Award Numbers: CNS2022-136156, FJC2020-043902-I; European Regional Development Fund, Grant/Award Number: PID2022-136478OB-C32; Gobierno de Aragon, Grant/Award Number: S74_23R; Next Generation EU, Grant/Award Numbers: CNS2022-136156, FJC2020-043902-IGrant PID2022-136478OB-C32 (MICIU/AEI/10.13039/501100011033, "ERDF A way of making Europe"). Grant CNS2022-136156 (MCIN/AEI/10.13039/501100011033, European Union Next Generation EU/PRTR). Gobierno de Aragon S74_23R research group. Grant FJC2020-043902-I(MCIN/AEI/10.13039/501100011033, 'Next- GenerationEU/PRTR').

Analysis of institutional authors

Nadal, MiquelCorresponding AuthorPeguero-Pina, Jose JavierAuthorSancho-Knapik, DomingoAuthorGil-Pelegrin, EustaquioAuthor

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May 14, 2025
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Comparison of different methods to evaluate tissue damage in response to leaf dehydration in Quercus ilex L. and Q. faginea Lam

Publicated to:Physiologia Plantarum. 177 (2): e70178- - 2025-03-01 177(2), DOI: 10.1111/ppl.70178

Authors: Nadal, Miquel; Peguero-Pina, Jose Javier; Sancho-Knapik, Domingo; Gil-Pelegrin, Eustaquio

Affiliations

CSIC, Estn Expt Aula EEAD, Zaragoza, Spain - Author
Ctr Invest & Tecnol Agroalimentaria Aragon CITA, Dept Sistemas Agr Forestales & Medio Ambiente, Zaragoza, Spain - Author
Univ Lorraine, AgroParisTech, INRAE, UMR Silva, Nancy, France - Author
Univ Zaragoza, Inst Agroalimentario Aragon IA2, CITA, Zaragoza, Spain - Author

Abstract

Determination of the point of critical damage in plant organs is crucial to elucidate the causes of plant mortality, but the different methodologies to quantify such damage have not been previously compared under the same experimental conditions. Here, we tested different indicators to evaluate damage in leaves of Quercus faginea and Q. ilex; in the latter case, 1- and 2-year-old leaves were included. The damage indicators were relative electrolyte leakage (REL), rehydration capacity (evaluated as the percentage loss of rehydration capacity; PLRC), chlorophyll fluorescence (maximum quantum yield of PSII; F-v/F-m), and the viability marker triphenyltetrazolium chloride (TTC). These damage indicators were evaluated in different sets of detached leaves for each species and leaf age dehydrated on the lab bench. Electrolyte leakage and PLRC showed a gradual response to decreasing relative water content, whereas F-v/F-m and TTC showed a threshold-like response, especially in the case of Q. faginea. Electrolyte leakage and TTC did not show differences between species and/or leaf ages. Measurement of F-v/F-m in dehydrating leaves proved to be the most straightforward, rapid and precise method for damage quantification, allowing for the differentiation in dehydration tolerance between Q. ilex and Q. faginea.

Keywords

Cell-membrane stabilityChlorophyll fluorescenceDaño vegetalDeshidrataciónDesiccation-toleranceDroughtElectrolitosElectrolyte leakageFluorescencia de clorofilaLeavesMortalidadÓrganos vegetativos de las plantasPhotosystem-iiQuercus fagineaQuercus ilexRehidrataciónSensitivitStressTriphenyltetrazolium chloride

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Physiologia Plantarum due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2025, it was in position 27/265, thus managing to position itself as a Q1 (Primer Cuartil), in the category Plant Sciences. Notably, the journal is positioned above the 90th percentile.

Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-07-12:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 2.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 2 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 1.5.
  • The number of mentions on the social network X (formerly Twitter): 1 (Altmetric).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

Leadership analysis of institutional authors

This work has been carried out with international collaboration, specifically with researchers from: France.

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (Nadal Nadal, Miquel) and Last Author (Gil Pelegrín, Eustaquio).

the author responsible for correspondence tasks has been Nadal Nadal, Miquel.