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May 7, 2025
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Article
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DNA-Based Nanocarriers to Sequester Altered microRNAs in Cardiac Dysfunction

Publicated to:Advanced Therapeutics. 7 (12): - 2024-12-01 7(12), DOI: 10.1002/adtp.202400247

Authors: Postigo, Alejandro; Hernandez-Bellido, Natalia; Sanchez-Barat, Marcos; Garcia-Mendivil, Laura; Pueyo, Esther; del Barrio, Jesus; Hernandez-Ainsa, Silvia; Ordovas, Laura

Affiliations

Ctr Invest Biomed Red Bioingn Biomat & Nanomed CIB - Author
Gobierno Aragon, Fdn ARAID - Author
Inst Invest Sanitaria IIS Aragon - Author
Univ Zaragoza, Inst Nanociencia & Mat Aragon INMA, CSIC - Author
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Abstract

MicroRNAs (miRs) play a critical role in modulating gene expression across biological processes, including cardiac aging and disease. As such, miRs have demonstrated therapeutic potential in several cardiac conditions. Efficient delivery of miR therapies to cardiac tissue is crucial for effective gene therapy and DNA-based nanocarriers (DNCs), based on Watson-Crick-Franklin highly specific base-pair recognition, have emerged as a promising, biocompatible alternative to viral-based methods. Here, DNCs designed to modulate miR levels as a potential treatment for cardiac dysfunction are presented. Specifically, the DNCs target miR-24-2, which inhibits SERCA2 gene. In humans, the reduction of SERCA2 activity is a hallmark of heart failure and is altered in cardiac aging. The assembled DNCs bearing anti-miR-24-2-5p sequences effectively restore intracellular levels of SERCA2 in a HEK293 cell model. The DNCs proper assembly is thoroughly verified, while their stability and miR-capture ability are demonstrated in vitro. The DNCs exhibit successful internalization into HEK293 and modest uptake into human cardiomyocytes. SERCA2 restoration by DNCs is significantly influenced by the miR-capture sequence layout, underscoring the importance of precise design for optimal biological outcomes. This study highlights the potential of DNCs in cardiac therapies, a previously unexplored avenue for addressing cardiac dysfunction.

Keywords

ArticleBase pairingCancerCardiac dysfunctionCardiac muscle cellCell viabilityControlled studyDeliveryDnaDna nanotechnologyGeneGene expressionGene therapyGood health and well-beingHeart failureHeart tissueHek293 cell lineHumanHuman cellIn vitro studyInduced pluripotent stem cellInternalization (cell)Massive expansionMicrornaMicrorna 24Microrna 24 2Microrna 24 2 5pMicrorna therapyMirnaNanocarrierNanohydrogelsNanotechnologySerca2 geneToehold-mediated strand displacementUnclassified drug

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Advanced Therapeutics due to its progression and the good impact it has achieved in recent years, according to the agency Scopus (SJR), it has become a reference in its field. In the year of publication of the work, 2024 there are still no calculated indicators, but in 2023, it was in position , thus managing to position itself as a Q1 (Primer Cuartil), in the category .

Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-11-22:

  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 9 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

    It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

    • The work has been submitted to a journal whose editorial policy allows open Open Access publication.
    Continuing with the social impact of the work, it is important to emphasize that, due to its content, it can be assigned to the area of interest of ODS 3 - Good Health and Well-Being, with a probability of 67% according to the mBERT algorithm developed by Aurora University.