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This research was co-financed by the European Union's Connecting Europe Facility (CEF) Grant No. INEA/CEF/ICT/A2018/1837816 GRAPEVINE project (hiGh peRformAnce comPuting sErvices for preVentIon and coNtrol of pEsts in fruit crops).

Analysis of institutional authors

Garces-Claver, AnaAuthorFayos, OretoAuthor

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December 11, 2024
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Molecular Detection of the Grapevine Pathogens Plasmopara viticola and Erysiphe necator from Airborne Inoculum Collector Cyclones

Publicated to:Agronomy-Basel. 14 (11): 2619- - 2024-11-01 14(11), DOI: 10.3390/agronomy14112619

Authors: Balduque-Gil, Joaquin; Garces-Claver, Ana; Perez-Lamuela, Ines; Barriuso-Vargas, Juan J; Fayos, Oreto

Affiliations

Agrifood Res & Technol Ctr Aragon CITA, Dept Plant Sci, Ave Montanana 930, Zaragoza 50059, Spain - Author
Int Ctr Adv Agron Mediterranean Studies CIHEAM Zar, Mediterranean Agron Inst Zaragoza, Ave Montanana 1005, Zaragoza 50059, Spain - Author
Univ Zaragoza, AgriFood Inst Aragon IA2, CITA, Zaragoza 50013, Spain - Author
Univ Zaragoza, Dept Agr Sci & Nat Environm, Ave Miguel Servet 177, Zaragoza 50013, Spain - Author

Abstract

Grapevine (Vitis vinifera L.) varieties are particularly susceptible to the pathogens downy mildew Plasmopara viticola and powdery mildew Erysiphe necator. Conventional methods for identifying and classifying spores rely on time-consuming microscopic examinations susceptible to human error and requiring qualified personnel. The aim of the present work has focused on the establishment of a protocol for the rapid molecular detection of the fungal species P. viticola and E. necator from adhesive tapes used to trap spores in airborne inoculum collector cyclones. Four DNA extraction methods were tested. Subsequently, molecular detection of both pathogens was performed by validating some of the specific molecular markers available in the literature. PCR with the primers Nad9 cob-F/Nad9 cob-R and Uncin144/Uncin511 showed specific results for P. viticola and E. necator, respectively, and the best results were obtained with the T-CTAB method. The methodology developed in this work could be of great help for relating direct measurement of P. viticola and E. necator airborne inoculum to disease risk and detection of pathogens, which could be integrated into the early diagnosis of these grapevine pathogens, improving existing warning systems such as Decision Support Systems.

Keywords

AssayDecision-support-systemsDetecciónDnDna extractionEarly detectionEsporasGestión de lucha integradaIntegrated pest managementMarcadores genéticosMolecular markersPcrPolymerase-chain-reactionPowdery mildewQuantificationRiskSporeSpores

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Agronomy-Basel due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2024 there are still no calculated indicators, but in 2023, it was in position 21/129, thus managing to position itself as a Q1 (Primer Cuartil), in the category Agronomy.

Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-08-06:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 10.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 9 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 2.5.
  • The number of mentions on the social network X (formerly Twitter): 4 (Altmetric).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.
  • Assignment of a Handle/URN as an identifier within the deposit in the Institutional Repository: http://hdl.handle.net/10532/7367

Leadership analysis of institutional authors

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: Last Author (Fayos Avellán, Oreto).