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Spanish Ministry of Science and Innovation(MCIN); European Union (EU)NextGenerationEU: PRTR-C17.I1,Grant/Award Number: AGROALNEXT;MCIN; State Research Agency (AEI),Grant/Award Number:PID2022-138484OR-I00; EU; Governmentof Aragon: "Grupo de investigacion enFruticultura - Caracterizacion, Adaptacion yMejora Genetica", Grant/Award Number:A12_23R; Spanish Ministry of Science,Innovation and Universities (MCIU); AEI,Grant/Award Number: I.M-L. predoctoralcontrac

Analysis of institutional authors

Medina-Lozano, InesAuthorBertolín, Juan RamónAuthorDiaz, AuroraCorresponding Author

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November 14, 2024
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Studies of genetic diversity and genome-wide association for vitamin C content in lettuce (Lactuca sativa L.) using high-throughput SNP arrays

Publicated to:Plant Genome. 17 (4): e20518-e20518 - 2024-10-24 17(4), DOI: 10.1002/tpg2.20518

Authors: Medina-Lozano, Ines; Bertolin, Juan Ramon; Plieske, Joerg; Ganal, Martin; Gnad, Heike; Diaz, Aurora

Affiliations

Agrifood Res & Technol Ctr Aragon CITA, Dept Anim Sci, Zaragoza, Spain - Author
Agrifood Res & Technol Ctr Aragon CITA, Dept Plant Sci, Avd Montanana 930, Zaragoza 50059, Spain - Author
SGS Inst Fresenius GmbH TraitGenet Sect, Seeland, Germany - Author
Univ Zaragoza, AgriFood Inst Aragon IA2, CITA, Zaragoza, Spain - Author

Abstract

Lettuce (Lactuca sativa L.) is a source of beneficial compounds though they are generally present in low quantities. We used 40K Axiom and 9K Infinium SNP (single nucleotide polymorphism) arrays to (i) explore the genetic variability in 21 varieties and (ii) carry out genome-wide association studies (GWAS) of vitamin C content in21 varieties and a population of 205 plants from the richest variety in vitamin C ('Lechuga del Pirineo'). Structure and phylogenetic analyses showed that the group formed mainly by traditional varieties was the most diverse, whereas the red commercial varieties clustered together and very distinguishably apart from the rest. GWAS consistently detected, in a region of chromosome 2, several SNPs related to dehydroascorbic acid (a form of vitamin C) content using three different methods to assess population structure, subpopulation membership coefficients, multidimensional scaling, and principal component analysis. The latter detected the highest number of SNPs (17) and the most significantly associated, 12 of them showing a high linkage disequilibrium with the lead SNP. Among the 84 genes in the region, some have been reported to be related to vitamin C content or antioxidant status in other crops either directly, like those encoding long non-coding RNA, several F-box proteins, and a pectinesterase/pectinesterase inhibitor, or indirectly, like extensin-1-like protein and endoglucanase 2 genes. The involvement of other genes identified within the region in vitamin C levels needs to be further studied. Understanding the genetic control of such an important quality trait in lettuce becomes very relevant from a breeding perspective.

Keywords

Ácido ascórbicoAscorbic acidDiversidad genética (como recurso)Estudios de asociación del genoma completoGenetic variationGenome, plantGenome-wide association studyLactucaLactuca sativaLinkage disequilibriumLocMarkersPhylogenyPolimorfismo de un solo nucleótidoPolymorphism, single nucleotideRevealsSoftwareTraitsTranscriptome

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Plant Genome due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2024 there are still no calculated indicators, but in 2023, it was in position 45/191, thus managing to position itself as a Q1 (Primer Cuartil), in the category Genetics & Heredity.

Independientemente del impacto esperado determinado por el canal de difusión, es importante destacar el impacto real observado de la propia aportación.

Según las diferentes agencias de indexación, el número de citas acumuladas por esta publicación hasta la fecha 2025-07-12:

  • Europe PMC: 1

Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-07-12:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 8.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 11 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 2.
  • The number of mentions on the social network X (formerly Twitter): 4 (Altmetric).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.

Leadership analysis of institutional authors

This work has been carried out with international collaboration, specifically with researchers from: Germany.

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (Medina Lozano, Inés) and Last Author (Díaz Bermúdez, Aurora).

the author responsible for correspondence tasks has been Díaz Bermúdez, Aurora.