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Grant support

This study was partially financed by an INIA-CITA agreement, FITE funds (LACTOCYNARA), European Union Regional Development funds, and the Research Group Funds of the Aragon Government (Ref. A06_20R and A14_20R). Yasemin Oner was supported by TUBITAK from Turkiye grant, n degrees: BIDEB-2219-1059B19170062266).

Análisis de autorías institucional
Iguacel L.p. - Autor o Coautor
Juan T. - Autor o Coautor
Calvo J.h. - Autor (correspondencia)
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Publicaciones > Artículo

Genome-Wide Association Studies of Somatic Cell Count in the Assaf Breed

Publicado en:Animals. 11 (6): - 2021-06-01 11(6), doi: 10.3390/ani11061531

Iguacel L.p.; Juan T.; Calvo J.h.;

Afiliaciones

A06_20RA14_20R European Union Regional Development funds - Financiador
A06_20RA14_20R INIA-CITA agreement - Financiador
A06_20RA14_20R TUBITAK from Turkiye - Financiador
ARAID, Zaragoza 50018, Spain - Autor o Coautor
BCCInnovation, Basque Culinary Ctr, Technol Ctr Gastron, Donostia San Sebastian 20009, Spain - Autor o Coautor
BIDEB-2219-1059B19170062266 FITE funds (LACTOCYNARA) - Financiador
BIDEB-2219-1059B19170062266 Research Group Funds of the Aragon Government - Financiador
Centro de Investigación y Tecnología Agroalimentaria de Aragón - Entidad de origen
INIA, Dept Mejora Genet Anim, Madrid 28040, Spain - Autor o Coautor
Uludag Univ, Dept Anim Sci, TR-16059 Bursa, Turkey - Autor o Coautor
Zaragoza Univ, Ctr Invest & Tecnol Agroalimentaria Aragon, Inst Agroalimentario Aragon IA2, Unidad Prod & Sanidad Anim,CITA, Zaragoza 50059, Spain - Autor o Coautor
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Resúmen

Resúmen: Simple Summary Mastitis causes economic loss due to discarded milk and reduced milk production and quality, increased medical care costs and somatic cell count (SCC) penalties. The use of genetic markers associated with the variability of this trait through marker-assisted selection (MAS) could help traditional methods. Our objectives were to identify new single nucleotide polymorphisms (SNPs) and genes associated with mastitis resistance in Assaf sheep by using the Illumina Ovine Infinium (R) HD SNP BeadChip (680K). Firstly, corrected phenotype estimates for somatic cell score (SCS) were calculated using 6173 records from 1894 multiparous Assaf ewes, and were used to select 192 extreme animals (low SCS group: n = 96; and high SCS group: n = 96) for the genome-wide association study (GWAS). Four SNPs (rs419096188, rs415580501, rs410336647, and rs424642424), three of them totally linked, were found to be significant at the chromosome level (FDR 10%) in two different regions of OAR19 close to genes related to the immune system response. Validation studies of two SNPs (rs419096188 and rs424642424) by Kompetitive Allele-Specific PCR (KASP) genotyping in the total population (n = 1894) confirmed previous GWAS association results for the SCS trait. Finally, the SNP rs419096188 was also associated with lactose content trait. A genome-wide association study (GWAS) was performed to identify new single nucleotide polymorphisms (SNPs) and genes associated with mastitis resistance in Assaf sheep by using the Illumina Ovine Infinium HD SNP BeadChip (680K). In total, 6173 records from 1894 multiparous Assaf ewes with at least three test day records and aged between 2 and 7 years old were used to estimate a corrected phenotype for somatic cell score (SCS). Then, 192 ewes were selected from the top (n = 96) and bottom (n = 96) tails of the corrected SCS phenotype distribution to be used in a GWAS. Although no significant SNPs were found at the genome level, four SNPs (rs419096188, rs415580501, rs410336647, and rs424642424) were significant at the chromosome level (FDR 10%) in two different regions of OAR19. The SNP rs419096188 was located in intron 1 of the NUP210 and close to the HDAC11 genes (61 kb apart), while the other three SNPs were totally linked and located 171 kb apart from the ARPP21 gene. These three genes were related to the immune system response. These results were validated in two SNPs (rs419096188 and rs424642424) in the total population (n = 1894) by Kompetitive Allele-Specific PCR (KASP) genotyping. Furthermore, rs419096188 was also associated with lactose content.

Palabras clave: dairy; gwas; mastitis; sheep; Dairy; Deacetylases; Genes; Gwas; Haplotypes; Mastitis; Mastitis resistance; Prediction; Reveals; Score; Sheep; Somatic cell count; Tool

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Best Categ.Agriculture, Dairy & Animal ScienceVeterinary (Miscellaneous)
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