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This research was funded by the Spanish Ministerio de Economia y Competitividad (MINECO), projects AGL2010-15694 and AGL2014-59171-R. J.T. is funded by a Juan de la Cierva-Incorporacion grant (IJC2018-035036-I).

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VviUCC1 Nucleotide Diversity, Linkage Disequilibrium and Association with Rachis Architecture Traits in Grapevine

Publicado en:Genes. 11 (6): - 2020-06-01 11(6), doi: 10.3390/genes11060598

Grimplet, Jerome;

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Resúmen: Cluster compactness is a trait with high agronomic relevance, affecting crop yield and grape composition. Rachis architecture is a major component of cluster compactness determinism, and is a target trait toward the breeding of grapevine varieties less susceptible to pests and diseases. Although its genetic basis is scarcely understood, a preliminary result indicated a possible involvement of the VviUCC1 gene. The aim of this study was to characterize the VviUCC1 gene in grapevine and to test the association between the natural variation observed for a series of rachis architecture traits and the polymorphisms detected in the VviUCC1 sequence. This gene encodes an uclacyanin plant-specific cell-wall protein involved in fiber formation and/or lignification processes. A high nucleotide diversity in the VviUCC1 gene promoter and coding regions was observed, but no critical effects were predicted in the protein domains, indicating a high level of conservation of its function in the cultivated grapevine. After correcting statistical models for genetic stratification and linkage disequilibrium effects, marker-trait association results revealed a series of single nucleotide polymorphisms (SNPs) significantly associated with cluster compactness and rachis traits variation. Two of them (Y-984 and K-88) affected two common cis-transcriptional regulatory elements, suggesting an effect on phenotype via gene expression regulation. This work reinforces the interest of further studies aiming to reveal the functional effect of the detected VviUCC1 variants on grapevine rachis architecture.

Palabras clave: Association genetics; Berry size; Bunch; Cluster compactness; Genes; Genome; Growth-regulators; Identification; Inflorescence; Pairwise relatedness; Phytocyanins; Plant; Rachis lignification; Single nucleotide polymorphism (snp); Table grape; Targeted sequencing; Uclacyanins; Vitis vinifera l; Vitis vinifera l.; Vitis viniferal

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