Análisis de autorías institucional
Tejedor-Calvo E - Autor (correspondencia)
Marco P - Autor o Coautor
Sanchez S - Autor o Coautor
Garcia-Barreda S - Autor o Coautor
Publicaciones > Artículo

Screening of bioactive compounds in truffles and evaluation of pressurized liquid extractions (PLE) to obtain fractions with biological activities

Publicado en:Food Research International. 132 (109054): 109054- - 2020-06-01 132(109054), doi: 10.1016/j.foodres.2020.109054

Tejedor-Calvo E; Marco P; Sanchez S; Garcia-Barreda S;


Centro de Investigación y Tecnología Agroalimentaria de Aragón - Entidad de origen
UAM, Inst Food Sci Res, Dept Prod & Characterizat Novel Foods, CSIC,CIAL, C Nicolas Cabrera 9,Campus Cantoblanco, Madrid 28049, Spain - Autor o Coautor
Univ Fed Parana, Dept Biochem & Mol Biol, Campus Ctr Politecn,CP 19046, BR-81531980 Curitiba, Parana, Brazil - Autor o Coautor
Universidad Autónoma de Madrid - Autor o Coautor
Universidad de Zaragoza - Autor o Coautor
Universidade Federal do Paraná - Autor o Coautor
Zaragoza Univ, CITA, Agrifood Inst Aragon IA2, Agrifood Res & Technol Ctr Aragon CITA,Dept Fores, Av Av Montanana 930, Zaragoza 50059, Spain - Autor o Coautor
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Resúmen: © 2020 Elsevier Ltd Truffles, besides the appreciated aromatic compounds, contain other molecules with interesting bioactive properties. A screening of fungal sterols and β-glucans within different truffle species and locations was carried out. These compounds were extracted with pressurized liquids (PLE) generating enriched fractions. Extraction efficiency was studied with a full-factorial experimental design (Response surface methodology, RSM), using water and ethanol as extraction solvents. Polysaccharides from truffle powder (TP) and the optimal PLE extract (EP) obtained were precipitated and analysed by NMR and GC–MS. THP-1 cell cultures were utilized to test immunomodulatory properties. With the optimal PLE conditions (16.7 MPa, 180 °C, 30 min) 64 and 22.5% yields were obtained respect, with water and ethanol, generating fractions containing respect, 9.1% β-glucan and 4.5% ergosterol. NMR analyses detected (1 → 3)-β-glucan structures in truffle. The EP induced a reduction of 40% IL-1β and 60% IL-6 pro-inflammatory cytokines secretion suggesting potential immunomodulatory activity.

Palabras clave: cytokines; ergosterol; immunomodulation; response surface methodology; tuber melanosporum; ?-d-glucans; Agaricus-bisporus; Beta-d-glucans; Chemical-composition; Cytokines; Edible mushrooms; Electron-beam; Enriched fractions; Ergosterol; Gamma-irradiation treatments; Immunomodulation; Nutritional-value; Quantification; Response surface methodology; Tuber; Tuber melanosporum; Β-d-glucans

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