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Grant support

The authors wish to express their gratitude to David Martinez and Daniel Fernandez de Luco (Universidad de Zaragoza), for providing samples. The Authors thank Sandro Pelini for providing assistance in the production of the GIS map. This research was supported by ANIHWA/Era-NET funds granted to Bru-Epidia project. Work at CITA-Spain was also sustained by Grants from MINECO (AGL2014-58795-C4-3R) and Aragon Government (Grupo de Investigacion A13-17D). Work at National and OIE Reference laboratory for Brucellosis, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. Caporale was also supported by the project IZSAM02/17RC from the Italian Ministry of Health ricerca corrente 2017 funds.Work at ANSES- France was supported by European Union Reference Laboratory for Brucellosis.

Análisis de autorías institucional
Maria Munoz, Pilar - Autor o Coautor
Jesus De Miguel, Maria - Autor o Coautor
Andres-Barranco, Sara - Autor o Coautor
Maria Blasco, Jose - Autor o Coautor
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Publicaciones > Artículo

Phylogeography and epidemiology of Brucella suis biovar 2 in wildlife and domestic swine

Publicado en:Veterinary Microbiology. 233 68-77 - 2019-06-01 233(), doi: 10.1016/j.vetmic.2019.04.025

Maria Munoz, Pilar; Jesus De Miguel, Maria; Andres-Barranco, Sara; Maria Blasco, Jose;

Afiliaciones

ANIHWA/Era-NET - Financiador
ANSES Paris Est Univ, EU OIE FAO, 14 Rue Pierre & Marie Curie, Maisons Alfort, France - Autor o Coautor
ANSES Paris Est Univ, Natl Reference Lab Brucellosis, Hlth Anim Lab, 14 Rue Pierre & Marie Curie, Maisons Alfort, France - Autor o Coautor
ANSES, Res & Reference Div, Strategy & Programmes Dept, 14 Rue Pierre & Marie Curie, Maisons Alfort, France - Autor o Coautor
APHA, 66 Ty Glas Rd, Cardiff CF14 5ZB, S Glam, Wales - Autor o Coautor
Aragon Government (Grupo de Investigacion) [A13-17D] - Financiador
Centro de Investigación y Tecnología Agroalimentaria de Aragón - Entidad de origen
Departamento de Patología Animal, Universidad de Zaragoza, Zaragoza, Spain. - Autor o Coautor
EU/OIE/FAO & National Reference Laboratory for Brucellosis, Animal Health Laboratory, ANSES / Paris-Est University, 14 rue Pierre et Marie Curie, Maisons-Alfort, France. - Autor o Coautor
European Union Reference Laboratory for Brucellosis - Financiador
Ist Zooprofilatt Sperimentale Abruzzo & Molise G, Natl & OIE Reference Lab Brucellosis, Teramo, Italy - Autor o Coautor
Ist Zooprofilatt Sperimentale Piemonte Liguria &, Lab Patol Anim & Stabulario, Turin, Italy - Autor o Coautor
Italian Ministry of HealthMinistry of Health, Italy [IZSAM02/17RC] - Financiador
Laboratorio Patologia Animale e Stabulario, Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta "I. Altara", Torino, Italy. - Autor o Coautor
MINECO [AGL2014-58795-C4-3R] - Financiador
National and OIE Reference laboratory for Brucellosis, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise "G. Caporale", Teramo, Italy. - Autor o Coautor
National and OIE Reference laboratory for Brucellosis, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise "G. Caporale", Teramo, Italy. Electronic address: g.garofolo@izs.it. - Autor o Coautor
Unidad de Producción y Sanidad Animal, Instituto Agroalimentario de Aragón (IA2), Centro de Investigación y Tecnología Agroalimentaria de Aragón (CITA)-Universidad de Zaragoza, Zaragoza, Spain. - Autor o Coautor
Univ Lyon, VetAgro Sup, ANSES Lyon Lab, UMR Mycoplasmoses Ruminants, Lyon, France - Autor o Coautor
Univ Lyon, VetAgro Sup, ANSES Lyon Lab, UMR Mycoplasmoses Ruminants, Marcy Letoile, France - Autor o Coautor
Univ Zaragoza, Ctr Invest & Tecnol Agroalimentaria Aragon CITA, IA2, Unidad Prod & Sanidad Anim, Zaragoza, Spain - Autor o Coautor
Univ Zaragoza, Dept Patol Anim, Zaragoza, Spain - Autor o Coautor
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Resúmen

Resúmen: Swine brucellosis due to Brucella suis biovar 2 (bv2) is enzootic in wild boar and hare in continental Europe and may cause major economic losses to the pig industry, mainly in free-ranged pig farms. The high nucleotide identity found among the B. suis biovar 2 isolates has long hindered the full understanding of the epidemiology and the phylogeography of the disease. Here, we used multilocus variable-number tandem-repeat (VNTR analysis (MLVA) and whole-genome analysis to identify single-nucleotide polymorphisms (SNPs) in order to gait insights from the largest B. suis bv2 dataset analyzed so far composed of domestic pigs and wildlife isolate collected throughout Europe since the 1970s. We found four major clades with a specific phylogeographic pattern. The Iberian Glade contains isolates exclusively from the Iberian Peninsula. The Central European clad includes most isolates from France, Northern Italy, Switzerland and an important proportion of those of Northern Spain. The Eastern European Glade clustered isolates from Croatia and Hungary mainly but also from areas of France, Germany, Italy and Poland. Finally, a separated Sardinian Glade grouped three isolates from this island At fine scale, MLVA demonstrated an endemic status of the infection in Europe and it allowed tracking a large outbreak formed by different farms from Spain linked to the same infection source. The whole genome SNPs analysis showed that the strains form genetically distinct clades, shared between wild boar and pigs, in agreement with the MLVA clades. Interestingly, all hare isolates clustered together within two groups compose exclusively of wildlife isolates. Our results support the hypothesis that maintenance and spread of B. suis bv2 in Europe is a dynamic process linked to the natural expansion of wild boar as the main wild reservoir of the infection, while spread over long distances is found largely dependent on anthropogenic activities.

Palabras clave: brucella suis; brucellosis; mlva; phylogeography; swine; Abortus; Boar sus-scrofa; Brucella suis; Brucellosis; Differentiation; Infection; Locus; Mlva; Multiplex pcr; Phylogeography; Polymorphism; Populations; Relatedness; Sequence; Swine; Wgs

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