Indexado en

Licencia y uso

Citaciones

Cited 6 times in

Cited 6 times in

Cited 4 times in

Altmetrics

Grant support

MT is the recipient of a pre-doctoral contract (FPI) by the CEU-UCH. AG-M and JQ are supported by a "Ramon y Cajal" contract of the Spanish Ministry of Science, Innovation and Universities (RYC2021-032245-I; RYC-2018-024985-I). This work was funded and approved by the grant for carrying out research, development and innovation projects by emerging research groups 2020 (GVA/2020/026), Generalitat Valenciana (Spain). Also, by the project from Spanish Ministry of Science and Innovation (Project reference PID2020-119462RA-I00/AEI/10.13039/501100011033).

Análisis de autorías institucional

Compartir

Description of the vaginal microbiota in nulliparous ewes during natural mating and pregnancy: preliminary signs of the male preputial microbiota modulation

Publicado en:Frontiers In Microbiology. 14 1224910- - 2024-01-11 14(), DOI: 10.3389/fmicb.2023.1224910

Autores: Barba, Marta; Toquet, Marion; Garcia-Rosello, Empar; Gomis, Jesus; Quereda, Juan J; Gonzalez-Torres, Pedro; Carbonetto, Belen; Gomez-Martin, Angel

Afiliaciones

Resumen

The vaginal microbiota plays a key role in animals' health. Understanding its diversity and composition and associated changes occurring through the reproductive cycle represents valuable knowledge to disclose the mechanisms leading to dysbiosis and eventually to infection. Even if the human vaginal microbiota has been thoroughly studied, scarce research has been conducted on the vaginal microbiota of livestock. In this study, 16S rRNA gene-based sequencing was performed on vaginal samples of ten nulliparous ewes at three different sampling points: before the estrus synchronization protocol (T0), at the time of estrus before mating (Testrus), and the day of the pregnancy diagnosis (Tpreg). Preputial samples from the three males collected pre and post-mating were also analyzed. Firmicutes, Proteobacteria, Bacteroidetes, and Actinobacteria were the most abundant phyla in vaginal samples. The most abundant genera were Porphyromonas, Anaerococcus, and Peptinophilius. Vaginal microbiota biodiversity decreased during pregnancy. Tenericutes (Ureaplasma spp.) increased significantly at Tpreg in both pregnant and non-pregnant ewes. Differences were observed between pregnant and non-pregnant ewes at Tpreg where pregnant ewes had a significantly higher abundance of Actinobacillus spp. and Ureaplasma spp. Ewes that were diagnosed with pregnancy at Tpreg showed a decreased abundance of gram-negative bacteria such as Bacteroidales, Campylobacterales, and Enterobacteriales. In addition, a significant decrease in the relative abundances of genera within Firmicutes, such as Alloicoccus (Lactobacillales), Atopostipes (Lactobacillales), and an uncultured bacteria W5053 from Family XI (Firmicutes, Clostridiales) was observed in non-pregnant ewes at Tpreg. The four most abundant phyla in the rams' prepuce were the same as in the ewes' vagina. The most abundant genus was Corynebacterium. No major differences were observed in the ram's preputial microbiota between pre and post-mating samples. Nevertheless, the differences in the taxonomic composition of ewes' vaginal microbiota between Testrus and Tpreg could be explained by the exposure to the preputial microbiota. This study offers new insights into the effects of several key steps of the ewe's reproductive cycle such as estrus-synchronization protocol, mating, and pregnancy on ovine vaginal microbiota. The knowledge of the microbiota dynamics during the reproductive cycle can help improve the reproductive outcomes of dams by identifying biomarkers and putative probiotics.

Palabras clave