{rfName}
Do

Indexed in

License and Use

Icono OpenAccess

Citations

19

Altmetrics

Analysis of institutional authors

Carvajal-Serna, MelissaAuthor

Share

February 4, 2025
Publications
>
Article
Sí

Does Melatonin Exert Its Effect on Ram Sperm Capacitation Through Nitric Oxide Synthase Regulation?

Publicated to: International Journal Of Molecular Sciences. 21 (6): 2093- - 2020-03-01 21(6), DOI: 10.3390/ijms21062093

Authors:

Miguel-Jimenez, Sara; Carvajal-Serna, Melissa; Calvo, Silvia; Casao, Adriana; Cebrian-Perez, Jose Alvaro; Muino-Blanco, Teresa; Perez-Pe, Rosaura
[+]

Affiliations

Univ Zaragoza, Fac Vet Sci, Dept Biochem & Mol & Cell Biol, Inst Environm Sci Aragon IUCA, Miguel Servet 177, Zaragoza 50013, Spain - Author

Abstract

Nitric oxide (NO), synthesized from L-arginine by nitric oxide synthase (NOS), is involved in sperm functionality. NOS isoforms have been detected in spermatozoa from different species, and an increment in NOS activity during capacitation has been reported. This work aims to determine the presence and localization of NOS isoforms in ram spermatozoa and analyse their possible changes during in vitro capacitation. Likewise, we investigated the effect of melatonin on the expression and localization of NOS and NO levels in capacitated ram spermatozoa. Western blot analysis revealed protein bands associated with neuronal NOS (nNOS) and epithelial NOS (eNOS) but not with inducible NOS (iNOS). However, the three isoforms were detected by indirect immunofluorescence (IFI), and their immunotypes varied over in vitro capacitation with cAMP-elevating agents. NO levels (evaluated by DAF-2-DA/PI staining) increased after in vitro capacitation, and the presence of L-arginine in the capacitating medium raised NO production and enhanced the acrosome reaction. Incubation in capacitating conditions with a high-cAMP medium with melatonin modified the NOS distribution evaluated by IFI, but no differences in Western blotting were observed. Melatonin did not alter NO levels in capacitating conditions, so we could infer that its role in ram sperm capacitation would not be mediated through NO metabolism.
[+]

Keywords

Acrosome reactionAnimalsCapacitationHuman spermatozoaInduced oxidative stressL-arginineLocalizatioMaleMelatoniMelatoninMotilityNitric oxideNitric oxide synthase type iNitric oxide synthase type iiiPromotes capacitationProteinRam spermatozoaSheepSperm capacitationSpermatozoaTyrosine phosphorylationViability

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2020, it was in position 67/295, thus managing to position itself as a Q1 (Primer Cuartil), in the category Biochemistry & Molecular Biology.

Independientemente del impacto esperado determinado por el canal de difusión, es importante destacar el impacto real observado de la propia aportación.

Según las diferentes agencias de indexación, el número de citas acumuladas por esta publicación hasta la fecha 2026-04-02:

  • WoS: 7
  • Scopus: 7
  • Europe PMC: 4
[+]

Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2026-04-02:

  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 21 (PlumX).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.
[+]

Awards linked to the item

This research was funded by CICYT AGL-2017-83799-R and DGA A07 17R. S.M.-J. has a predoctoral contract from the Diputacion General de Aragon (DGA).
[+]