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This work has been funded by the projects PID2021-122153NA-I00 from the Spanish Ministry of Science and Innovation to PRI and StG-757886-ELONGAN from the European Research Council to PBA.

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Carvajal-Serna, MelissaAuthor

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February 4, 2025
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MEK signalling pathway is required for hypoblast specification and migration in ovine

Publicated to:Reproduction. 167 (6): e240003- - 2024-06-01 167(6), DOI: 10.1530/REP-24-0003

Authors: de los Reyes, Nuria Martinez; Flores-Borobia, Ines; Carvajal-Serna, Melissa; Marigorta, Pilar; Bermejo-Alvarez, Pablo; Ramos-Ibeas, Priscila

Affiliations

CSIC, INIA, Dept Anim Reprod, Madrid, Spain - Author

Abstract

Early embryo development requires the differentiation of three cell lineages in two differentiation events. The second lineage specification differentiates the inner cell mass into epiblast, which will form the proper fetus, and hypoblast, which together with the trophectoderm will form the extraembryonic membranes and the fetal part of the placenta. MEK signalling pathway is required for hypoblast differentiation in mouse embryos, but its role in ungulate embryos remains controversial. The aim of this work was to analyse the role of MEK signalling on hypoblast specification at the blastocyst stage and on hypoblast migration during post-hatching stages in vitro in the ovine species. Using well-characterized and reliable lineage markers, and different MEK inhibitor concentrations, we demonstrate that MEK signalling pathway is required for hypoblast specification in the inner cell mass of the ovine blastocyst, and that it plays a role during the hypoblast migration occurring following blastocyst hatching. These results show that the role of MEK signalling pathway on hypoblast specification is conserved in phylogenetically distant mammals.

Keywords

AnimalsBlastocystBlastocyst inner cell massBovineCdx2Cell differentiationCell lineageCell movementCell-proliferationEmbryonic developmentEpiblastExpressionFemaleFgfInhibitorsMap kinase signaling systemMiceOct-PregnancyRolesSegregationSheepSignal transduction

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Reproduction due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2024 there are still no calculated indicators, but in 2023, it was in position 6/39, thus managing to position itself as a Q1 (Primer Cuartil), in the category Developmental Biology.

Independientemente del impacto esperado determinado por el canal de difusión, es importante destacar el impacto real observado de la propia aportación.

Según las diferentes agencias de indexación, el número de citas acumuladas por esta publicación hasta la fecha 2025-08-06:

  • WoS: 1
  • Scopus: 2
  • Europe PMC: 2

Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-08-06:

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 2.
  • The number of mentions on the social network X (formerly Twitter): 2 (Altmetric).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.