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Grant support

This work was supported by the Ministry of Education, Youth and Sports of the Czech Republic within the program INTER-COST (LTC20004) and from the project TowArds Next GENeration Crops, reg. no. CZ.02.01.01/00/22_008/0004581 of the ERDF Programme Johannes Amos Comenius.

Analysis of institutional authors

Grimplet, JeromeAuthorCabetas, Maria Jose RubioAuthor

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December 26, 2024
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Article
Hybrid Gold

Genome editing in almond using hairy root transformation system

Publicated to:Plant Cell Tissue And Organ Culture. 159 (3): 74- - 2024-12-01 159(3), DOI: 10.1007/s11240-024-02935-x

Authors: Jedlickova, Veronika; Stefkova, Marie; Lopez, Juan Francisco Sanchez; Grimplet, Jerome; Cabetas, Maria Jose Rubio; Robert, Helene S

Affiliations

Ctr Invest & Tecnol Agroalimentaria Aragon CITA, Dept Ciencia Vegetal, Zaragoza 500 59, Spain - Author
Masaryk Univ, CEITEC MU Cent European Inst Technol, Mendel Ctr Plant Genom & Prote, Hormonal Crosstalk Plant Dev, Brno 62500, Czech Republic - Author
Masaryk Univ, Fac Sci, Natl Ctr Biomol Res, Brno 625 00, Czech Republic - Author
Univ Zaragoza, Inst Agroalimentario Aragon IA2, CITA, Zaragoza 50013, Spain - Author

Abstract

Woody plant species are often challenging to transform, which complicates gene function studies using molecular biology techniques. To develop an effective gene editing tool for almond (Prunus dulcis, syn. Prunus amygdalus), we established a new method for inducing hairy roots in almond seedlings based on the injection of agrobacterial culture. We generated composite plants comprising wild-type shoots and transgenic hairy roots. This approach was also successful in almond x peach hybrids. The hairy root transformation system was used alongside Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated protein 9 gene editing to target two transcription factor genes (ETHYLENE RESPONSE FACTOR 74 and GIBBERELLIC ACID INSENSITIVE). We successfully generated hairy roots with knockouts of the target genes and evaluated the efficiency of guide RNAs for gene editing in almond. Our transgenic root method could be a valuable tool for routine gene function studies in almond.

Keywords

<italic>prunus dulcis</italic>Ácido giberelicoAgrobacterium-mediated transformationArabidopsisComposite plantsCrispr/cas9Edición de genesEfficiencyErf74EtilenoGaGaiHairy root cultureMultiplexPrunus dulcisRaícesRepeticiones palindrómicas cortas agrupadas y regularmente interespaciadasRnStrategyToolTransformación genética

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Plant Cell Tissue And Organ Culture due to its progression and the good impact it has achieved in recent years, according to the agency Scopus (SJR), it has become a reference in its field. In the year of publication of the work, 2024 there are still no calculated indicators, but in 2023, it was in position , thus managing to position itself as a Q1 (Primer Cuartil), in the category Horticulture.

Independientemente del impacto esperado determinado por el canal de difusión, es importante destacar el impacto real observado de la propia aportación.

Según las diferentes agencias de indexación, el número de citas acumuladas por esta publicación hasta la fecha 2025-07-15:

  • WoS: 2
  • Scopus: 2

Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-07-15:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 1.

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 4.05.
  • The number of mentions on the social network X (formerly Twitter): 1 (Altmetric).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.
  • Assignment of a Handle/URN as an identifier within the deposit in the Institutional Repository: http://hdl.handle.net/10532/7432

Leadership analysis of institutional authors

This work has been carried out with international collaboration, specifically with researchers from: Czech Republic.