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This work was supported by Ministerio de Ciencia e Innovacion, Spain(PID2020-113239RB-100)and Gobierno de Aragon (A07_23R). VP-Dhasa predoctoral contract from the Diputacion General de Aragon (DGA).

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Noya, AgustiAuthorCarvajal-Serna, MelissaAuthor

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July 18, 2024
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Differential effect of melatonin on ram spermatozoa depending on the allelic variant of the RsaI polymorphism of the MTR1A gene, incubation medium and season

Publicated to:Reproduction Fertility And Development. 36 (10): RD23233- - 2024-01-01 36(10), DOI: 10.1071/rd23233

Authors: Pena-Delgado, Victoria; Noya, Agusti; Carvajal-Serna, Melissa; Abecia, Jose A; Perez-Pe, Rosaura; Casao, Adriana

Affiliations

Univ Zaragoza, Fac Vet, BIOFITER IUCA, Miguel Servet 177, Zaragoza 50013, Spain - Author

Abstract

Context. The RsaI polymorphism of the melatonin receptor MTNR1A gene affects seasonal reproduction in sheep, but its effect on ram spermatozoa and their response to melatonin is unknown. Aims. This study aims to evaluate whether RsaI polymorphism of the MTNR1A gene influences the response of ram spermatozoa to in vitro added melatonin. Methods. Spermatozoa from rams carrying different RsaI allelic variants were incubated with melatonin in a TALP medium or a capacitation-triggering medium during the reproductive and non-reproductive seasons. After incubation, sperm motility, membrane integrity, mitochondria activity, oxidative damage, apoptotic markers and capacitation status were assessed. Key results. In the reproductive season, the T/T genotype was related to some adverse effects of melatonin when spermatozoa were incubated in TALP medium, whereas the C/C genotype was linked with adverse effects when the hormone was added in a capacitation-triggering medium. The decapacitating effect of melatonin on spermatozoa was also different depending on genotype. Conclusions. The melatonin effect on spermatozoa from rams carrying different RsaI genotypes differed depending on the season and the medium. Implications. The knowledge of the RsaI allelic variant of the MTNR1A gene of rams could be helpful when carrying out in vitro reproductive techniques in the ovine species.

Keywords

1a mtnr1a geneAllelesAnimalsApoptosiAssociationCapacitationGenotypeIncubationMaleMelatoninMembrane integrityMt1Mtnr1a polymorphismPolymorphism, geneticRam spermReceptorReceptor, melatonin, mt1Reproductive seasonalityRsaiSeasonsSheeSheepSpermSperm capacitationSperm motilitySpermatozoa

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Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Reproduction Fertility And Development due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2024 there are still no calculated indicators, but in 2023, it was in position 24/181, thus managing to position itself as a Q1 (Primer Cuartil), in the category Zoology.

Independientemente del impacto esperado determinado por el canal de difusión, es importante destacar el impacto real observado de la propia aportación.

Según las diferentes agencias de indexación, el número de citas acumuladas por esta publicación hasta la fecha 2025-08-05:

  • WoS: 2
  • Scopus: 2

Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-08-05:

  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 5 (PlumX).