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This work was supported by JSPS KAKENHI Grant Numbers 15H02431, 19H00941 and 19J14774. We thank Lesley Benyon, Ph.D., from Edanz Group (https://en-author-services.edanzgroup.com/) for editing a draught of this manuscript.

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8 de septiembre de 2024
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Characterization of a gene co-expression network associated with MGST, the pollen modifier gene of gametophytic self-incompatibility in sweet cherry (Prunus avium L.)

Publicado en:Acta Horticulturae. 1312 9-15 - 2021-01-01 1312(), DOI: 10.17660/ActaHortic.2021.1312.2

Autores: Ono, K; Chang, E T; Nishiyama, S; Wunsch, A; Tao, R

Afiliaciones

CITA Univ Zaragoza, Inst Agroalimentario Aragon IA2, Zaragoza, Spain - Autor o Coautor
Ctr Invest & Tecnol Agroalimentaria Aragon CITA, Unidad Hortofruticultura, Avda Montahana 930, Zaragoza 50059, Spain - Autor o Coautor
Kyoto Univ, Grad Sch Agr, Lab Pomol, Kyoto 6068502, Japan - Autor o Coautor

Resumen

Most sweet cherry (Prunus avium L.) cultivars exhibit S-RNase-based gametophytic self-incompatibility ( SI), a major obstacle for fruit production. Clarifying the molecular mechanism behind the SI reaction will contribute to future improvements in sweet cherry productivity. Although the S haplotype combination of pollen and seed parent determines SI specificity in Prunus, as in other S-RNase-based SI plants, accumulated experimental results imply the presence of a Prunus-specific SI mechanism. The sweet cherry cultivar 'Cristobalina', with a functional S locus, shows pollen-part self-compatibility (SC) owing to the downregulated expression of an SI modifier in pollen. This modifier has been identified as M-locus-encoded glutathione Stransferase-like ( MGST), but it has not been well characterized. In this study, we investigated the transcriptomic profiles of pollen with relatively higher and lower MGST expression levels, M-pollen and m-pollen, respectively. 'Cristobalina' (Mm) F1 populations segregating for SC (Mm) and SI (MM), 'Cristobalina' selfed progeny of the mm genotype (SC), and diverse SI sweet cherry cultivars (MM) were used. Illumina mRNA-seq reads obtained from a pollen grain or germinated pollen tube of each sample were mapped to the sweet cherry reference-coding sequence. A differential expression analysis among the genotypic combinations yielded 985 and 853 differentially expressed genes in the pollen-grain and germinated pollen-tube stages, respectively; however, only MGST was detected in all the pollen-grain comparisons. This supported our hypothesis that MGST functions in the S-RNase activation of pollen. However, a differentially expressed gene clustering analysis and a weighted gene co-expression network analysis revealed significant correlations between MGST's expression and the expression levels of functional gene sets, such as those involved in lipid metabolism and stress responses. Thus, it is also possible that MGST may function in some pollen development- or viability-related processes. Further analyses are necessary to elucidate the molecular function of MGST in the Prunus-specific SI reaction.

Palabras clave

AlmonCloningDifferential gene expressionDuplicationsF-box geneGlutathione s-transferaseHaplotypesIdentificationLocusProteinS-rnaseS-rnasesSequenceTranscriptome analysiTranscriptome analysisWeighted gene co-expression network analysis

Indicios de calidad

Impacto bibliométrico. Análisis de la aportación y canal de difusión

El trabajo ha sido publicado en la revista Acta Horticulturae, Q4 Agencia Scopus (SJR), su enfoque regional y su especialización en Horticulture, le otorgan un reconocimiento lo suficientemente significativo en un nicho concreto del conocimiento científico a nivel internacional.

2025-07-06:

  • Scopus: 1

Impacto y visibilidad social

Desde la dimensión de Influencia o adopción social, y tomando como base las métricas asociadas a las menciones e interacciones proporcionadas por agencias especializadas en el cálculo de las denominadas “Métricas Alternativas o Sociales”, podemos destacar a fecha 2025-07-06:

  • La utilización de esta aportación en marcadores, bifurcaciones de código, añadidos a listas de favoritos para una lectura recurrente, así como visualizaciones generales, indica que alguien está usando la publicación como base de su trabajo actual. Esto puede ser un indicador destacado de futuras citas más formales y académicas. Tal afirmación es avalada por el resultado del indicador “Capture” que arroja un total de: 2 (PlumX).

Análisis de liderazgo de los autores institucionales

Este trabajo se ha realizado con colaboración internacional, concretamente con investigadores de: Japan.