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Grant support

The authors gratefully thank Maria Herrero and Eliseo Rivas for their helpful discussion and advice. This work was supported by the Ministerio de Economia y Competitividad - European Regional Development Fund, European Union [grant number BES-2010-037992 to E.F.]; the Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria [grant numbers RFP2015-00015-00, RTA2014-00085-00, RTA2017-00003-00]; and the Gobierno de Aragon - European Social Fund, European Union [Grupo Consolidado A12-17R].

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Fadon, Erica - Autor (correspondencia)
Rodrigo, Javier - Autor o Coautor
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Publicaciones > Artículo

Combining Histochemical Staining and Image Analysis to Quantify Starch in the Ovary Primordia of Sweet Cherry during Winter Dormancy

Publicado en:Jove-Journal Of Visualized Experiments. (145): - 2019-03-20 (145), doi: 10.3791/58524

Fadon, Erica; Rodrigo, Javier;

Afiliaciones

Centro de Investigación y Tecnología Agroalimentaria de Aragón - Entidad de origen
efadona@cita-aragon.es. - Autor o Coautor
Gobierno de Aragon - European Social Fund, European Union [Grupo Consolidado] [A12-17R] - Financiador
Instituto Agroalimentario de Aragón - IA2 (CITA-Universidad de Zaragoza), Centro de Investigación y Tecnología Agroalimentaria de Aragón - Autor o Coautor
Instituto Agroalimentario de Aragón - IA2 (CITA-Universidad de Zaragoza), Centro de Investigación y Tecnología Agroalimentaria de Aragón. - Autor o Coautor
Instituto Nacional de Investigacion y Tecnologia Agraria y AlimentariaInstituto de Investigacion Agropecuaria (INIA) [RFP2015-00015-00, RTA2014-00085-00, RTA2017-00003-00] - Financiador
Ministerio de Economia y Competitividad - European Regional Development Fund, European Union [BES-2010-037992] - Financiador
Univ Zaragoza, CITA, IA2, Ctr Invest & Tecnol Agroalimentaria Aragon, Zaragoza, Spain - Autor o Coautor
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Resúmen

Resúmen: Changes in starch in small structures are associated with key events during several plant developmental processes, including the reproductive phase from pollination to fertilization and the onset of fruiting. However, variations in starch during flower differentiation are not completely known, mainly due to the difficulty of quantifying the starch content in the particularly small structures of the flower primordia. Here, we describe a method for the quantification of starch in the ovary primordia of sweet cherry (Prunus avium L.) by using an image analysis system attached to the microscope, which allows relating the changes in starch content with the different phases of dormancy from autumn to spring. For this purpose, the dormancy status of flower buds is determined by evaluating the bud growth of shoots transferred to controlled conditions at different moments in winter time. For the quantification of starch in the ovary primordia, flower buds are sequentially collected, fixed, embedded in paraffin wax, sectioned, and stained with I(2)Kl (potassium iodide-iodine). Preparations are observed under the microscope and analyzed by an image analyzer that clearly distinguishes starch from the background. Starch content values are obtained by measuring the optical density of the image that corresponds to the stained starch, considering the sum of the optical density of each pixel as an estimation of the starch content of the frame studied.

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Best Categ.Multidisciplinary SciencesChemical Engineering (Miscellaneous)
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IF1.1630.570--10.600
Rank45/7091/271
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DD7D4-
TT2T2
PP37P34
Index ERIC-
Index Emergin-
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