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This review was made in the frame of projects PID2019-107601RB-C31 and PID2019-107601RA-C32 financed by MCIN/AEI/10.1303910.13039/501100011033. A.E-B's work was supported by a Ph.D. Fellowship funded by the Institute of Tropical Health University of Navarra (ISTUN) funders (Fundacion la CAIXA [LCF/PR/PR13/51080005], Fundacion Maria Francisca de Roviralta, and Ubesol) and REPRODIVAC project under Grant Agreement No. 10106081 through the HORIZON-CL6-2021-FARM2FORK-01-06 Call. P.M.M's (CITA) work was also supported by the Aragon Government (Grupo de Investigacion A21_23R).

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A Review on the Methodology and Use of the Pregnant Mouse Model in the Study of Brucella Reproductive Pathogenesis and Its Abortifacient Effect

Publicado en:Microorganisms. 12 (5): 866- - 2024-05-01 12(5), DOI: 10.3390/microorganisms12050866

Autores: Elizalde-Bielsa, Aitor; Munoz, Pilar M; Zuniga-Ripa, Amaia; Conde-Alvarez, Raquel

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Resumen

Brucellosis is one of the most common and widespread bacterial zoonoses and is caused by Gram-negative bacteria belonging to the genus Brucella. These organisms are able to infect and replicate within the placenta, resulting in abortion, one of the main clinical signs of brucellosis. Although the mouse model is widely used to study Brucella virulence and, more recently, to evaluate the protection of new vaccines, there is no clear consensus on the experimental conditions (e.g., mouse strains, doses, routes of inoculation, infection/pregnancy time) and the natural host reproducibility of the pregnant mouse model for reproductive brucellosis. This lack of consensus calls for a review that integrates the major findings regarding the effect of Brucella wild-type and vaccine strains infections on mouse pregnancy. We found sufficient evidence on the utility of the pregnant mouse model to study Brucella-induced placentitis and abortion and propose suitable experimental conditions (dose, time of infection) and pregnancy outcome readouts for B. abortus and B. melitensis studies. Finally, we discuss the utility and limitations of the pregnant mouse as a predictive model for the abortifacient effect of live Brucella vaccines.

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